Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping.
Identifieur interne : 000E96 ( Main/Exploration ); précédent : 000E95; suivant : 000E97Adsorption of DNA binding proteins to functionalized carbon nanotube surfaces with and without DNA wrapping.
Auteurs : Yu Ishibashi [Japon] ; Shusuke Oura [Japon] ; Kazuo Umemura [Japon]Source :
- European biophysics journal : EBJ [ 1432-1017 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, DNA-Binding Proteins, Nanotubes, Carbon, Polyethylene Glycols, Rec A Recombinases.
- Adsorption, Models, Molecular, Molecular Conformation, Surface Properties.
Abstract
We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH2 groups (CONH2-SWNT) exhibited very strong interactions between the CONH2-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.
DOI: 10.1007/s00249-017-1200-3
PubMed: 28204854
Affiliations:
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last="Oura">Shusuke Oura</name><affiliation wicri:level="1"><nlm:affiliation>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601</wicri:regionArea><wicri:noRegion>1628601</wicri:noRegion></affiliation></author><author><name sortKey="Umemura, Kazuo" sort="Umemura, Kazuo" uniqKey="Umemura K" first="Kazuo" last="Umemura">Kazuo Umemura</name><affiliation wicri:level="1"><nlm:affiliation>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601, Japan. meicun2006@163.com.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biophysics Section, Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, 1628601</wicri:regionArea><wicri:noRegion>1628601</wicri:noRegion></affiliation></author></analytic><series><title level="j">European biophysics journal : EBJ</title><idno type="eISSN">1432-1017</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adsorption</term><term>DNA (chemistry)</term><term>DNA-Binding Proteins (chemistry)</term><term>Models, Molecular</term><term>Molecular Conformation</term><term>Nanotubes, Carbon (chemistry)</term><term>Polyethylene Glycols (chemistry)</term><term>Rec A Recombinases (chemistry)</term><term>Surface Properties</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>Adsorption</term><term>Conformation moléculaire</term><term>Modèles moléculaires</term><term>Nanotubes de carbone ()</term><term>Polyéthylène glycols ()</term><term>Propriétés de surface</term><term>Protéines de liaison à l'ADN ()</term><term>Rec A Recombinases ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term><term>DNA-Binding Proteins</term><term>Nanotubes, Carbon</term><term>Polyethylene Glycols</term><term>Rec A Recombinases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adsorption</term><term>Models, Molecular</term><term>Molecular Conformation</term><term>Surface Properties</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Adsorption</term><term>Conformation moléculaire</term><term>Modèles moléculaires</term><term>Nanotubes de carbone</term><term>Polyéthylène glycols</term><term>Propriétés de surface</term><term>Protéines de liaison à l'ADN</term><term>Rec A Recombinases</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We examined the adsorption of DNA binding proteins on functionalized, single-walled carbon nanotubes (SWNTs). When SWNTs were functionalized with polyethylene glycol (PEG-SWNT), moderate adsorption of protein molecules was observed. In contrast, nanotubes functionalized with CONH<sub>2</sub> groups (CONH<sub>2</sub>-SWNT) exhibited very strong interactions between the CONH<sub>2</sub>-SWNT and DNA binding proteins. Instead, when these SWNT surfaces were wrapped with DNA molecules (thymine 30-mers), protein binding was a little decreased. Our results revealed that DNA wrapped PEG-SWNT was one of the most promising candidates to realize DNA nanodevices involving protein reactions on DNA-SWNT surfaces. In addition, the DNA binding protein RecA was more adhesive than single-stranded DNA binding proteins to the functionalized SWNT surfaces.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Ishibashi, Yu" sort="Ishibashi, Yu" uniqKey="Ishibashi Y" first="Yu" last="Ishibashi">Yu Ishibashi</name></noRegion><name sortKey="Oura, Shusuke" sort="Oura, Shusuke" uniqKey="Oura S" first="Shusuke" last="Oura">Shusuke Oura</name><name sortKey="Umemura, Kazuo" sort="Umemura, Kazuo" uniqKey="Umemura K" first="Kazuo" last="Umemura">Kazuo Umemura</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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